Epigenetic Re-Programming during Mammalian Preimplantation Embryogenesis and Pgc Development
نویسندگان
چکیده
Abbreviations: PGCs: Primordial Germ Cells; XCI: X Chromosome Inactivation; 5mC: 5-methylcytosine; 5hmC: 5-hydroxymethylcytosine; Xp: Paternal X Chromosome; 5fC: 5-formylcytosine; 5caC: 5-carboxylcytosine; ICM: Inner Cell Mass; PRC2: Polycomb Repressive Complex 2; IAPs: Intracisternal-A-Particles; CGIs: CpG Islands; gDMRs: Germline Differently Methylated Regions; Xi: Inactive X Chromosome; qRT-PCR: Quantitative Reverse Transcription Polymerase Chain Reaction
منابع مشابه
I-13: Transcriptome Dynamics of Human and Mouse Preimplantation Embryos Revealed by Single Cell RNA-Sequencing
Background: Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. However, it is still unclear about the crucial transcriptional network and key hub genes that regulate the proceeding of preimplantation embryos. Materials and Methods: Through single-cell RNAsequencing (RNA-seq) of both human and mouse preimplantation embryos, ...
متن کاملMethylation dynamics in the early mammalian embryo: implications of genome reprogramming defects for development.
In mouse and most other mammalian species, the paternal and maternal genomes undergo parent-specific epigenetic reprogramming during preimplantation development. The paternal genome is actively demethylated within a few hours after fertilization in the mouse, rat, pig, bovine, and human zygote, whereas the maternal genome is passively demethylated by a replication-dependent mechanism after the ...
متن کاملGerm cells from mouse and human embryonic stem cells.
Mammalian gametes are derived from a founder population of primordial germ cells (PGCs) that are determined early in embryogenesis and set aside for unique development. Understanding the mechanisms of PGC determination and differentiation is important for elucidating causes of infertility and how endocrine disrupting chemicals may potentially increase susceptibility to congenital reproductive a...
متن کاملEpigenetic regulation in mammalian preimplantation embryo development
Preimplantation embryo development involves four stages: fertilization, cell cleavage, morula and blastocyst formation. During these stages, maternal and zygotic epigenetic factors play crucial roles. The gene expression profile is changed dramatically, chromatin is modified and core histone elements undergo significant changes. Each preimplantation embryo stage has its own characteristic epige...
متن کاملGlobal profiling of DNA methylation erasure in mouse primordial germ cells.
Epigenetic reprogramming, characterized by loss of cytosine methylation and histone modifications, occurs during mammalian development in primordial germ cells (PGCs), yet the targets and kinetics of this process are poorly characterized. Here we provide a map of cytosine methylation on a large portion of the genome in developing male and female PGCs isolated from mouse embryos. We show that DN...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017